LOCUS       40924_46098        6762 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Envelope expression vector pVpack 10A1, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6762)
  AUTHORS   Vaillancourt P, Grafsky AJ.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-JAN-2001) Technical Services, Stratagene, 11011 N. 
            Torrey Pines Rd, La Jolla, CA 92037, USA
REFERENCE   2  (bases 1 to 6762)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 6762)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (09-JAN-2001) Technical Services, Stratagene, 11011 N. Torrey Pines 
            Rd, La Jolla, CA 92037, USA"
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6762
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             930..2867
                     /codon_start=1
                     /product="10A1 envelope protein"
                     /label=10A1 envelope protein
                     /protein_id="AAK01731.1"
                     /translation="MARSTLSKPLKDKINPWKSLMVMGVLLRVGMAESPHQVFNVTWRV
                     TNLMTGRTANATSLLGTVQDAFPRLYFDLCDLVGEEWDPSDQEPYVGYGCKYPGGRKRT
                     RTFDFYVCPGHTVKSGCGGPREGYCGEWGCETTGQAYWKPTSSWDLISLKRGNTPWDTG
                     CSKMACGPCYDLSKVSNSFQGATRGGRCNPLVLEFTDAGKKANWDGPKSWGLRLYRTGT
                     DPITMFSLTRQVLNIGPRIPIGPNPVITGQLPPSRPVQIRLPRPPQPPPTGAASIVPET
                     APPSQQPGTGDRLLNLVEGAYQALNLTNPDKTQECWLCLVSGPPYYEGVAVVGTYTNHS
                     TAPASCTATSQHKLTLSEVTGQGLCMGALPKTHQALCNTTQSAGSGSYYLAAPAGTMWA
                     CSTGLTPCLSTTMLNLTTDYCVLVELWPRIIYHSPDYMYGQLEQRTKYKREPVSLTLAL
                     LLGGLTMGGIAAGIGTGTTALIKTQQFEQLHAAIQTDLNEVEKSITNLEKSLTSLSEVV
                     LQNRRGLDLLFLKEGGLCAALKEECCFYADHTGLVRDSMAKLRERLNQRQKLFESGQGW
                     FEGQFNRSPWFTTLISTIMGPLIVLLLILLFGPCILNRLVQFVKDRISVVQALVLTQQY
                     HQLKPIEYEP"
     RBS             1898..1906
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     misc_feature    2908..3494
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     CDS             3514..4110
                     /codon_start=1
                     /label=PuroR
                     /note="puromycin N-acetyltransferase"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVECPKDRATWCMTRKPGA"
     polyA_signal    4255..4479
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(4605..4621)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(4629..4645)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4653..4683)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(4698..4719)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(5007..5595)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(5769..6626)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(6627..6731)
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gcttggtacc
      901 gagctcggat cgatatctgc ggccgcacca tggcgcgttc aacgctctca aaacccctta
      961 aagataagat taacccgtgg aagtccttaa tggtcatggg ggtcctatta agagtaggga
     1021 tggcagagag cccccatcag gtctttaatg taacctggag agtcaccaac ctgatgaccg
     1081 ggcgtaccgc caatgccacc tcccttttag gaactgtaca agatgccttc ccaagattat
     1141 attttgatct atgtgacctg gtcggagaag agtgggaccc ttcagaccag gaaccatatg
     1201 tcgggtatgg ctgcaaatac cccggaggga gaaagcggac ccggactttt gacttttacg
     1261 tgtgccctgg gcataccgta aaatcggggt gtggggggcc aagagagggc tactgtggtg
     1321 aatggggttg tgaaaccacc ggacaggctt actggaagcc cacatcatca tgggacctaa
     1381 tctcccttaa gcgcggtaac accccctggg acacgggatg ctccaaaatg gcttgtggcc
     1441 cctgctacga cctctccaaa gtatccaatt ccttccaagg ggctactcga gggggcagat
     1501 gcaaccctct agtcctagaa ttcactgatg caggaaaaaa ggctaattgg gacgggccca
     1561 aatcgtgggg actgagactg taccggacag gaacagatcc tattaccatg ttctccctga
     1621 cccgccaggt cctcaatata gggccccgca tccccattgg gcctaatccc gtgatcactg
     1681 gtcaactacc cccctcccga cccgtgcaga tcaggctccc caggcctcct cagcctcctc
     1741 ctacaggcgc agcctctata gtccctgaga ctgccccacc ttctcaacaa cctgggacgg
     1801 gagacaggct gctaaacctg gtagaaggag cctatcaggc gcttaacctc accaatcccg
     1861 acaagaccca agaatgttgg ctgtgcttag tgtcgggacc tccttattac gaaggagtag
     1921 cggtcgtggg cacttatacc aatcattcta ccgccccggc cagctgtacg gccacttccc
     1981 aacataagct taccctatct gaagtgacag gacagggcct atgcatggga gcactaccta
     2041 aaactcacca ggccttatgt aacaccaccc aaagtgccgg ctcaggatcc tactaccttg
     2101 cagcacccgc tggaacaatg tgggcttgta gcactggatt gactccctgc ttgtccacca
     2161 cgatgctcaa tctaaccaca gactattgtg tattagttga gctctggccc agaataattt
     2221 accactcccc cgattatatg tatggtcagc ttgaacagcg taccaaatat aagagggagc
     2281 cagtatcgtt gaccctggcc cttctgctag gaggattaac catgggaggg attgcagctg
     2341 gaatagggac ggggaccact gccctaatca aaacccagca gtttgagcag cttcacgccg
     2401 ctatccagac agacctcaac gaagtcgaaa aatcaattac caacctagaa aagtcactga
     2461 cctcgttgtc tgaagtagtc ctacagaacc gaagaggcct agatttgctc ttcctaaaag
     2521 agggaggtct ctgcgcagcc ctaaaagaag aatgttgttt ttatgcagac cacacgggac
     2581 tagtgagaga cagcatggcc aaactaaggg aaaggcttaa tcagagacaa aaactatttg
     2641 agtcaggcca aggttggttc gaagggcagt ttaatagatc cccctggttt accaccttaa
     2701 tctccaccat catgggacct ctaatagtac tcttactgat cttactcttt ggaccctgca
     2761 ttctcaatcg attggtccaa tttgttaaag acaggatctc agtggtccag gctctggttt
     2821 tgactcaaca atatcaccag ctaaaaccta tagagtacga gccatgataa ctgcaggtcg
     2881 agcatgcatc tagggcggcc aattccgccc ctctccctcc ccccccccta acgttactgg
     2941 ccgaagccgc ttggaataag gccggtgtgc gtttgtctat atgtgatttt ccaccatatt
     3001 gccgtctttt ggcaatgtga gggcccggaa acctggccct gtcttcttga cgagcattcc
     3061 taggggtctt tcccctctcg ccaaaggaat gcaaggtctg ttgaatgtcg tgaaggaagc
     3121 agttcctctg gaagcttctt gaagacaaac aacgtctgta gcgacccttt gcaggcagcg
     3181 gaacccccca cctggcgaca ggtgcctctg cggccaaaag ccacgtgtat aagatacacc
     3241 tgcaaaggcg gcacaacccc agtgccacgt tgtgagttgg atagttgtgg aaagagtcaa
     3301 atggctctcc tcaagcgtat tcaacaaggg gctgaaggat gcccagaagg taccccattg
     3361 tatgggatct gatctggggc ctcggtgcac atgctttaca tgtgtttagt cgaggttaaa
     3421 aaaacgtcta ggccccccga accacgggga cgtggttttc ctttgaaaaa cacgatgata
     3481 agcttgccac aacccacaag gagacgacct tccatgaccg agtacaagcc cacggtgcgc
     3541 ctcgccaccc gcgacgacgt cccccgggcc gtacgcaccc tcgccgccgc gttcgccgac
     3601 taccccgcca cgcgccacac cgtcgacccg gaccgccaca tcgagcgggt caccgagctg
     3661 caagaactct tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt cgcggacgac
     3721 ggcgccgcgg tggcggtctg gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc
     3781 gagatcggcc cgcgcatggc cgagttgagc ggttcccggc tggccgcgca gcaacagatg
     3841 gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt ggttcctggc caccgtcggc
     3901 gtctcgcccg accaccaggg caagggtctg ggcagcgccg tcgtgctccc cggagtggag
     3961 gcggccgagc gcgccggggt gcccgccttc ctggagacct ccgcgccccg caacctcccc
     4021 ttctacgagc ggctcggctt caccgtcacc gccgacgtcg agtgcccgaa ggaccgcgcg
     4081 acctggtgca tgacccgcaa gcccggtgcc tgacgcccgc cccacgaccc gcagcgcccg
     4141 accgaaagga gcgcacgacc ccatggctcc gaccgaagcc gacccgggcg gccccgccga
     4201 ccccgcaccc gcccccgagg cccaccgact ctagagctcg ctgatcagcc tcgactgtgc
     4261 cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag
     4321 gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta
     4381 ggtgtcattc tattctgggg ggtggggtgg ggcaggacag caagggggag gattgggaag
     4441 acaatagcag gcatgctggg gatgcggtgg gctctatggc ttctgaggcg gaaagaacca
     4501 gctggggctc gagtgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat
     4561 gtctgtatac cgtcgacctc tagctagagc ttggcgtaat catggtcata gctgtttcct
     4621 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
     4681 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
     4741 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
     4801 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
     4861 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
     4921 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     4981 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     5041 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     5101 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     5161 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc aatgctcacg ctgtaggtat
     5221 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     5281 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     5341 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     5401 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt
     5461 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     5521 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     5581 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     5641 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
     5701 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
     5761 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
     5821 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
     5881 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
     5941 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
     6001 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
     6061 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
     6121 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
     6181 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
     6241 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
     6301 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
     6361 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
     6421 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
     6481 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
     6541 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
     6601 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
     6661 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
     6721 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tc
//
